'Super-resolution microscopy at visible and near-infrared wavelengths: from nanomaterials to deep tissue imaging' with Professor Laurent Cognet, from the University of Bordeaux
Date: 22 January 2020 Time: 15:00 - 16:00
Abstract: Laurent Cognet
LP2N - Institut d’Optique, CNRS & University of Bordeaux, Talence, France
Sub-wavelength localization of single nano-emitters is the basis of several super-resolution microscopy approaches which are now applied in different fields of science requiring nanoscale imaging performance. In more complex systems than isolated nanostructures or living cells, it still remains a challenge to apply super-resolution imaging approaches, especially in thick intact samples. This is due to the limited brightness of fluorescent emitters, the optical aberrations induced by the samples and/or the poor penetration of the light into materials at visible wavelengths.
To circumvent these limitations, we recently developed several new approaches. More precisely, I will present SELFI, an original strategy to super-localise a single molecule in 3D which allowed 3D super-resolution microscopy to be achieved at unprecedented depths inside a biological tissue . I will then present another approach based on single carbon nanotube tracking in the near-infrared  to obtain super-resolved maps of the extracellular space within intact live brain tissues . Finally I will show that a novel family of single molecule nanoprobes can be engineered in the near-infrared through the creation of photoswitchable carbon nanotubes  and ultrashort carbon nanotubes displaying localized emission centers that could be reveal by super-resolution microscopy of the nanotubes themselves .
 Bon et al Nat. Methods. (2018), Linares-Loyez et al, Front. Phys. (2019)
 Fakhri, et al Science (2010), Danné, et al ACS Photonics (2018)
 Godin, et al Nat. Nanotechnol. (2017), Paviolo, et al Methods (2019)
 Godin, et al Adv. Science (2019)
 Cognet et al Nanoletters (2008), Danné et al, ACS Nano, (2018), Mandal et al, in revision
Bio: after completing his PhD in atom optics at the Institut d’Optique supervised by A. Aspect (Paris Sud University, Orsay), Professor Laurent Cognet's early experiments focused on the detection and tracking of single fluorescent molecules in living cells as a postdoc in Th. Schmidt's group at Leiden University (NL).
In 2000, he was tenured by CNRS as junior researcher at University of Bordeaux and he was promoted Research Director in 2009.
In 2011, he participated in the creation of the "Laboratoire Photonique Numérique et Nanosciences" (LP2N-Institut d'Optique-CNRS at University of Bordeaux) where is deputy director and coordinates the ?Optics and Biology Research Initiative.
|Location:||Lecture Theatre PP2, People's Palace, Mile End Campus|
|Contact:||Dr Matteo Palma|